[Automated-sequencing] Slab Gel v Capillary Sequencing - Question from an economist

O'Connor, Alan C. oconnor at rti.org
Fri May 12 11:31:43 EST 2006


Hello, I am an economist working on a study of the transition from slab
gel (ABI 377) to capillary array sequencing (ABI 3730 or MegaBACE 1000).
I have reviewed the literature, but have some basic questions about the
amount of time it took to use a slab gel instrument. Can someone help? 

 

Here are my questions:

How long did it take to mix, prepare, pour, and set up the ABI 377? I
know this is supposed to be very time consuming, but I haven't been able
to find out how long it actually took. I'm interested in number of man
hours involved - meaning the actual manual time which excludes the time
needed for the gel to set.

 

How long did it take to do the post-run lane retracking? This is
supposed to be really frustrating, but I would like to know about how
long it took to do it for an ABI 377 with 96 lanes.

 

Thanks!!

Alan

 

 

Alan O'Connor

RTI International

28 Second Street, Suite 300

San Francisco, CA  94105

 

(415) 538-0871

(415) 538-0879 (fax)

oconnor at rti.org

 



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