[Automated-sequencing] 3 prime sequencing & library construction

Fulton, Ciaran ciaran.Fulton at almac-diagnostics.com
Tue May 23 07:49:36 EST 2006


I'm interested in 3'-prime sequencing of cDNA libraries. Has anyone any experience of how successful this is, any personal experience or any papers. I've heard of processes whereby the polyadenylated tail can be modified to allow using dT anchoring primer, does any one know of any commercially available kits etc.

As always, any advice would be gratefully appreciated.

Best regards,


Ciaran


Dr. Ciaran Fulton
Team Leader
Sequencing R&D Group

Almac Diagnostics Ltd
19 Seagoe Industrial Estate
Craigavon 
Co Armagh
BT63 5QD
Northern Ireland

Tel ('): +44 (0)28 38 39 57 22
Fax (7):  +44 (0)28 38 39 86 76
Email (*): ciaran.fulton at almac-diagnostics.com


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