[Automated-sequencing] DNA SEQ: 3730 Tray on Deck errors
Phillip San Miguel
pmiguel at purdue.edu
Tue Sep 5 11:24:36 EST 2006
[Just to widen the pool of 3730 users, this is a cross post to ABRF
forum and bionet.genome.autosequencing]
There has been some prior discussion of a particular error that has
bedeviled our facility more and more of late:
The error is "Tray on deck does not match tray type in run setup".
For us, this generally occurs between the A1 and B1 quads of a 384
sequencing reaction plate. Generally, once it occurs, we are shut down
until and engineer fixes it. We have two 3730XL's and have actually seen
this on both instruments during the last month. The fix is generally for
the ABI Field Service Engineer to replace all the sensors in the gripper
Usually this seems to do the trick--for a time.
The FSE will almost universally warn us that sensors can be damaged
by liquids being spilled on them. But then just as universally note that
there was no evidence that this had occurred with our sensors. (The
sensors are dry and have no buffer residue on them.)
The situation is somewhat more complex than I'm describing
here--frequently this error seems to presage the failure of some major
system of a 3730. But not always.
Has anyone else been seeing sensor issues in their 3730's?
Purdue Genomics Core Facility
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