[Automated-sequencing] DNA SEQ: 3730 Tray on Deck errors

Phillip San Miguel via autoseq%40net.bio.net (by pmiguel from purdue.edu)
Fri Sep 14 14:59:41 EST 2007


Butz, Malinda L. wrote:
> Phillip:
> 
> You posted a message on ABRF about a year ago concerning some ABI 3730
> errors.  In your reply e-mail you mentioned a specific error
> "Autosampler serial port communication failed"  Did you ever figure out
> what was causing this error message?  Was it a default ground wire?  Any
> help you can give would be greatly appreciated.
> 
> Thank you,
> 
> Malinda
> 
> [...]

	My major Autosampler problem arose because I had changed the default 
FTP root folder location on the console computer for reasons that need 
not concern us here. As a result, a calibration file that the sequencer 
grabs and uses from the default FTP root folder was not being updated by 
ABI service technicians during calibrations. So, no matter what 
calibrations they did, they would only last until the next time I 
restarted the machine.

	All my other autosampler issues resulted from sensors burning out. The 
fix for this, other than just replacing sensors as they fried, was to 
buy new waste/buffer/water container lids with fresh, tight gaskets. 
That prevented liquids from dripping onto the autosampler.

	That is all that comes to mind, anyway.

Phillip



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