From s.lautenschlaeger from labor-brunner.de  Tue Nov 11 09:11:00 2008
From: s.lautenschlaeger from labor-brunner.de (Sabine Lautenschlaeger)
Date: Tue Nov 11 12:28:57 2008
Subject: [Automated-sequencing] ALf express
Message-ID: <20081111141732.8D17A3F46E@mailout1.mk-netzdienste.de>

Hi,

 

I?m also sequencing PCR-Products using Alf Express.

 

I clean the PCR-Product from the old primers using the ?PCR-kit? from GE. 

This product is used directly without any ss-ends.

Instead of cy5-dATP I have sequencing primers 5?labeled with cy5.

The sequencing primer are positioned like a ?nested PCR-primer? a little bit
away from the end of the PCR product.

The sequencing reaction is carried out using the ?Sequencing-Kit? from GE.

If you are interested in the protokoll, please let me know.

 

Saluti and ciao

 

Sabine Lautenschl?ger