[Automated-sequencing] quality scores using phred from MegaBACE esd
(by agonzalez from gemabiotech.com)
Tue Nov 16 07:45:00 EST 2010
Hi good morning.
I'm user of MegaBACE 1000 DNA sequencer, my sequences using
MegaBACE1000pack software v 3.1 runing in Windows XP ServicePck3. The
problem that Ihave an interphace soft (CodonCode corp.) to change score
quality megabace to score phred doesn't run in this operative windows.
-Any suggest about how I have to do with the analyzed .esd files from
MegaBACE 1000 to change to score phred?
-there's another program or software to do this and compatible to run in
Windows XP Srvice pck3?
I can manage and use this .esd files to see sequence and make consensus
analysis, but we need get really phred score in all fragments sequences.
Thnks a lot for any help
Lic. Alex González
El 22/10/2009 13:09, Sven Klages escribió:
> hat I'm new to these packages and therefore apologize if i'm
> submitting an obvious question.
> I have received a set off .SFF files that I would like to process/align.
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