Organizing sequencing projects

Roy Smith roy at phri.nyu.edu
Mon Jan 22 10:25:45 EST 1990


	I'm working on some software to help organize large sequencing
projects and have some questions.  When embarking on a big project these
days, what is the general strategy?  Is it still shotgun clone into M13 and
try to piece the fragments together afterwards?  If so, how would you
describe the fragment (i.e. readable sequence, excluding the primer) sizes
you get; is uniformly distributed in [50,350] a reasonable first guess?

	Once you have the fragment sequences on line, what tools are
commonly used to find the overlaps and assemble them into a composite
sequence?
-- 
Roy Smith, Public Health Research Institute
455 First Avenue, New York, NY 10016
{att,philabs,cmcl2,rutgers,hombre}!phri!roy -or- roy at alanine.phri.nyu.edu
"The connector is the network"




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