Oligos

Gary Williams x3294 gwilliam at mrc-crc.ac.uk
Wed Jan 31 10:19:03 EST 1990


Does anyone know of a program to aid the selection of regions of a
nucleic acid sequence to act as good primers for PCR?

Ideally, the regions required should have a high Td so that they do not
anneal to themselves.  They should form stable duplexes with and be
specific to, the target sequence.  They should not base-pair to other
regions on the target sequence.


Gary Williams

Computing Services Section,		 Janet:       G.Williams at UK.AC.CRC
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