SEQUENCE MODIFYING BUG IN MACMOLLY/ENTER&LIST V.3.5.1

Heikki Lehv{slaiho Heikki.Lehvaslaiho at Helsinki.FI
Mon Apr 6 04:15:59 EST 1992


We just found out an annoying and potentially serious bug in MacMolly
sequence analysis package version 3.5.1 (the latest one, version 4 should
be out soon). 

In the program Enter&List the command "Edit Reverse Complement" (this command
is not available prior version 3.5.)  doesn't work correctly. If the
selection contains an odd number of nucleotides it forgets to convert the
middle one; e.g. if the original sequence is CCCCCCC, the "reverse complement" 
command will change it to GGGCGGG! This holds for all sequences with an odd 
number of nucleotides (e.g. 3, 21, 2001), but not with even numbers. This 
problem does not occur when using the "File Save as reverse complementary" 
command. 

We've informed the authors at Soft Gene GmbH about this bug.

Here at the Meilahti Departments several PCR oligos were modified by this
bug. If you've ever used this command, you better check out your sequences!

        	-Heikki

Heikki Lehv{slaiho  <Heikki.Lehvaslaiho at Helsinki.FI>
Meilahti Medical Departments, University of Helsinki
Haartmaninkatu 3, SF-00290 Helsinki, FINLAND
Phone: +358-0-434 6408         FAX: +358-0-434 6491




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