NCSA GelReader 2.0.3

Jennie File jfile at ncsa.uiuc.edu
Thu Sep 17 11:44:20 EST 1992


NCSA GelReader v2.0.3 has been released!  It is available via anonymous 
FTP (ftp.ncsa.uiuc.edu or 141.142.20.50) in the Mac/GelReader directory; 
via the archive server (archive-server at ncsa.uiuc.edu); or via the 
Technical Resource Catalog. Catalogs may be obtained by contacting:

 NCSA
 University of Illinois at Urbana-Champaign
 Attention: Documentation Orders
 152 Computing Applications Building
 605 E. Springfield Ave.
 Champaign, IL 61820

Please send any questions or comments to softdev at ncsa.uiuc.edu 
and any bug reports to bugs at ncsa.uiuc.edu.

The following is a copy of the README file:

Congratulations on your acquisition of NCSA GelReader 2.0.3 for the 
Macintosh! Note that there are two different versions of this 
program: one for machines that have not floating point processor, 
and one for machines that do.

NCSA GelReader is a product designed to automate to some degree 
the measurement of DNA length using digitized electrophoretic gels. 
First, a digitized gel image is read into GelReader, and displayed as an 
image. GelReader has the capability to find the lanes and the bands 
in the gel. Lanes and bands can be interactively added and deleted if 
necessary. Then, known molecular weights (standards) can be used 
to approximate the molecular weight of the remaining molecules. The 
result is a text report listing the approximated molecular weights of 
the molecules.

To get started, you will need to open the UnStuffit application to 
decompress the GelReader application, documentation, and sample 
files.

NEW FEATURES IN VERSION 2.0

This version has been completely rewriten, and now provides a much 
more intuitive user interface. Creating, deleting and editing 
standards is now much easier, and assigning standards to lanes in a 
gel has been greatly improved.

Profiles now appear in the same window with the gel. You may 
superimpose several profiles in the same view. You can also save a 
gel file, its' lanes and bands and its notebook into an HDF file. When 
you open the HDF file again, you will see all the lanes and bands that 
were there when you saved it, and the notebook will still be intact.

Multiplexing is also supported now. You can run standards and 
unknowns in the same lane, probe for the knowns and generate the 
image, then probe for the unknowns and generate a separate image. 
The results of the analysis of the image with the knowns can then be 
superimposed onto the image with the unknowns.

And much, much more.

FIXES
  in 2.0.2:

1) There was a bug that would cause the program to crash whenever
     you manually added more than one lane. Fixed!
2) It no longer reports standards.
3) It would sometimes report than the program was out of memory
    when using the copy lanes command. Fixed.
4) It no longer crashes when you try to generate a report and you
    have unknowns above internal controls (knows imbedded in the
    lane.
5) Computations for unknowns outside of the range of the knowns
    are still less accurate, but tend to be much more reasonable now,
    and the fact that these computation are less accurate are flagged 
    in the report.

  in 2.0.3:

1) it no longer refreshes the entire screen when deleting or adding
    bands.
2) I can no longer reproduce the file save problems, although I don't
    know why.

WHAT THIS DISK CONTAINS

GelReader2.0.3LCsi.sit	compressed GelReader application(No FPU)
	or
GelReader2.0.3mac.sit	compressed GelReader application
Samples.sit	compressed sample files
README	this file
UnStuffit 1.51		decompression utility

Thomas Redman
Project Leader
National Center for Supercomputing Appls.
152 Computing Applications Building
Champaign, IL  61820




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