[Q]IBM program to calc oligo pmol?

Bob DeLisio delisior at rnisd0.DNET.roche.com
Thu Aug 12 18:07:58 EST 1993


Bob (Belas at mbimail.umd.edu) writes:

>        Does anyone know of a shareware or freeware program that calculates
>the pmolarity of an oligonucleotide give sequence and OD260?  Any program
>that can allow easy calcs of this sort would do.  Thanks.


I simply use the formula:

micromolarity = OD260 units x Dilution Factor x 30 micrograms
		---------------------------------------------
	((A x 312.2) + (G x 328.2) + (C x x288.2) + (T x 303.2) - 61.0)

where A=# of A's in an oligo, etc.

Of course, micromolarity = picomoles/microliter

Hope that helps,
Bob
=====================================================
 _/   _/     Robert DeLisio
  _/ _/      Roche Institute of Molecular Biology
    _/       Roche Research Center
   _/ _/     Nutley, New Jersey 07110-1199
  _/   _/
   _/ _/     Voice: (201) 235-3728
     _/      Fax:   (201) 235-2839
    _/ _/    Internet: delisior at rnisd0.dnet.roche.com
   _/   _/   CompuServe: 73517,2616
=====================================================





More information about the Bio-soft mailing list