Oligonucleotide Tm prediction

Dan Jacobson danj at welchgate.welch.jhu.edu
Fri Feb 5 14:43:38 EST 1993


In article <1993Feb5.122833.1 at hkucc.hku.hk> vldnaseq at hkucc.hku.hk writes:
>Dear netters,
>
>I am optimizing the hybridization and washing conditions for single base
>mismatch oligonucleotide hybridization experiments.  This is used as a means
>to detect mutations.  Is there any software that can predict accurately the
>Tm of a complete match / single mismatch under different ionic strengths ?
>
>Wai Ming

A quick Gopher seacrh tells me that there is a program for the PC
called Melt (by John Nash) which may be what you're looking for.

You can obtain melt.uue by gopher or by anonymous ftp at
felix.embl-heidelberg.de.

Best of luck,

Dan Jacobson

danj at welchgate.welch.jhu.edu




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