Lucas, et al primer software?
hamel at ccu.umanitoba.ca
Thu Jul 29 09:10:30 EST 1993
Does anybody have any experience with the primer design program
described in following article? I'm interested in trying it out but would
appreciate any comments (costs only fifty US $).
TI: An improved microcomputer program for finding gene- or gene family-
specific oligonucleotides suitable as primers for polymerase chain reactions
or as probes.
AU: Lucas-K; Busch-M; Mossinger-S; Thompson-JA
AD: Institut fur Immunbiologie, Universitat Freiburg, FRG.
SO: Comput-Appl-Biosci. 1991 Oct; 7(4): 525-9
AB: We present here an easy-to-use computer program which finds
oligonucleotides suitable as primers in polymerase chain reactions (PCR) or
as probes for hybridization. In contrast to other programs used for this
purpose, the additional advantage of this one is the possibility of directly
detecting gene- as well as gene family-specific oligonucleotides. For this
purpose, up to 200 different DNA sequences, of maximally 65,000 nucleotides
each, can be scanned in a single search to ensure either single or multiple
gene binding of the PCR primers or probes. Specific oligonucleotides for genes
carrying internal repetitions and for single genes belonging to a set of
highly conserved genes can also be detected. Many parameters such as
exclusion of simple sequences, which are known to be highly repeated
throughout various genomes or regions of stable secondary structures in both
primer-primer and primer-template, can be taken into consideration and
avoided. Furthermore, the G + C content and the length of the
oligonucleotides can be changed in a broad range by the user.
Andre Hamel email: hamel at ccu.umanitoba.ca
Manitoba Veterinary Services lab tel.: (204) 945-7630
Infectious & Genetic Disease FAX: (204) 945-8062
Mol.Biol.Lab., Winnipeg, Manitoba, CANADA
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