Lucas, et al primer software?

Andre Hamel hamel at ccu.umanitoba.ca
Thu Jul 29 09:10:30 EST 1993


	Does anybody have any experience with the primer design program
described in following article? I'm interested in trying it out but would
appreciate any comments (costs only fifty US $).

TI: An improved microcomputer program for finding gene- or gene family-
specific oligonucleotides suitable as primers for polymerase chain reactions 
or as probes.
AU: Lucas-K; Busch-M; Mossinger-S; Thompson-JA
AD: Institut fur Immunbiologie, Universitat Freiburg, FRG.
SO: Comput-Appl-Biosci. 1991 Oct; 7(4): 525-9
ISSN: 0266-7061
PY: 1991
LA: ENGLISH
CP: ENGLAND
AB: We present here an easy-to-use computer program which finds 
oligonucleotides suitable as primers in polymerase chain reactions (PCR) or 
as probes for hybridization. In contrast to other programs used for this 
purpose, the additional advantage of this one is the possibility of directly 
detecting gene- as well as gene family-specific oligonucleotides. For this 
purpose, up to 200 different DNA sequences, of maximally 65,000 nucleotides 
each, can be scanned in a single search to ensure either single or multiple 
gene binding of the PCR primers or probes. Specific oligonucleotides for genes 
carrying internal repetitions and for single genes belonging to a set of 
highly conserved genes can also be detected. Many parameters such as 
exclusion of simple sequences, which are known to be highly repeated 
throughout various genomes or regions of stable secondary structures in both 
primer-primer and primer-template, can be taken into consideration and 
avoided. Furthermore, the G + C content and the length of the 
oligonucleotides can be changed in a broad range by the user.

thanks

                            ********************
Andre Hamel                                 email: hamel at ccu.umanitoba.ca
Manitoba Veterinary Services                lab tel.: (204) 945-7630
Infectious & Genetic Disease                     FAX: (204) 945-8062     
Mol.Biol.Lab., Winnipeg, Manitoba, CANADA
                            ********************




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