A colleaque told me he'd heard of a program that predicts primers for use in APPCR/RAPD - based on genome size GC/AT content etc. Does anyone know anything about this? Apparently it was a *bar* conversation! Thanks if you can shed any light on this. Jon Clewley VRD, CPHL, Colindale, London, UK NW9 5HT jclewley at crc.ac.uk