Plasmid construction simulation.

hosun at ALLIANT.SNU.AC.KR hosun at ALLIANT.SNU.AC.KR
Fri Apr 15 23:24:13 EST 1994


Hello, Netters.

Is there any one heard of the program manuplate plasmid sequence?

I want to make a program simulate plasmid manuplation. 
display restriction fragments and plasmid map.
make result plasmid sequence. and so on..

like Restriction with EcoRI and elute small fragmet.
     and then Blunting with Klenow fragment of DNA Pol.
     and ligating with SmaI digested vector.
then what result is expected?
Is this stratagy valid?

any advice is welcome.
thanx in advance.
 
Son Hosun
Mol. Bio.
Seoul National Univ.
hosun at alliant.snu.ac.kr




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