Plasmid construction simulation.

Enrico V. Cantu chembb at
Mon Apr 18 23:22:25 EST 1994

hosun at ALLIANT.SNU.AC.KR wrote:
: Hello, Netters.

: Is there any one heard of the program manuplate plasmid sequence?

: I want to make a program simulate plasmid manuplation. 
: display restriction fragments and plasmid map.
: make result plasmid sequence. and so on..

: like Restriction with EcoRI and elute small fragmet.
:      and then Blunting with Klenow fragment of DNA Pol.
:      and ligating with SmaI digested vector.
: then what result is expected?
: Is this stratagy valid?

: any advice is welcome.
: thanx in advance.

I'm not so sure about the Klenow frag. manipulation, but definitely 
MacPlasMap can do the manipulation of gene deletions, restriction digests, 
fusions, etc.  Like it's name implies, it's available for the Macintosh,
is small, and comes with standard vectors already.  It is available via
anonymous ftp from several sites, including indiana.
Enrico Cantu  Internet: ECantu at  NeXTMail: enricoc at 
"Dear Lord, forgive me for the sin of arrogance, and Lord, by arrogance,
I mean the following..."

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