dot readers

Christopher Blencowe cbb at convex.phazc.uni-heidelberg.de
Fri Aug 19 08:45:42 EST 1994


Ron Lundstrom (ron at bullwinkle.cric.com) wrote:


:   Many experiments consist of gridding clones in rectangular arrays
: and hybridizing probes against the array. This results in digital
: images consisting of arrays of dots.

:   I am looking for dot readers, i.e., software that reads a digital
: image of a dot blot and reads the positive dots. I am aware of
: two packages, DOTS 8.0 from Argonne National Labs and a dot
: reader from HELIX. I also know about NIH Image.

:   I am interested in both automatic and interactive programs.

:   I really want to try all the packages I can, free and commercial,
: since the software may be used by several research groups in my 
: company. Since we will be using a pooling scheme on our grids,
: it would be nice if the package were extensible or programable to
: decipher the pooling.

: Anyone have any suggestions or leads ?

: %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
: % Ron Lundstrom  Collaborative Research Inc.  617-487-7979 x148  %
: % ron at cric.com        1365 Main Street        617-891-5062 (fax) %
: %                    Waltham, MA. 02154                          %
: %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%


There is a Shareware one called 'counter' , which is not to specfic.
I can send it to you by EMail.

It will let you specify the areas of a dig. image, and will
count the intensity of the grey-scale of the specified area.

Maybe if you have only black/white this will be what you want.

Great program, simple to handle, MS-DOS / WIN3.1  needed.

No experience, but I will try this (if I get a CCD camera).

Low Cost (10$US)

Sincerly, Christopher





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