Reading DNA sequences from scanned pictures

Brian Foley brianf at med.uvm.edu
Fri Aug 26 15:27:54 EST 1994


Priyal A. De Zoysa (now at Royal Free) (pdezoysa at crc.ac.uk) wrote:
: I wish to read sequences into a PC (Windows/DOS) from a scanned 
: image of the sequencing gel 
: autoradiograph.  Does anyone know of any reasonably priced 
: (or free) software that will enable me
: to do this.

I don't know what the price is, but the July 1994 (volume 17, number 1)
issue of BioTechniques has an ad on page 197 (in the New Products section)
for DNAscan by ISS-Enprotech (circle number 339 on the reader service card).

It claims to be 99% accurate, but I don't see how they can make a blanket
claim like that because accuracy will depend on how fuzzy your bands
are and how good your scanner or camera is, etc.  Maybe they mean it
was 99% as accurate as an average human reading the same gel?

It says it is Windows-based, and can take images from a CCD camera, flatbead
scanners, hand-held scanners and phosphorimagers.

Good luck.  Let us know if you find anything that really works.

--
********************************************************************
*  Brian Foley               *     If we knew what we were doing   *
*  Molecular Genetics Dept.  *     it wouldn't be called research  *
*  University of Vermont     *                                     *
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