Reamplification of the RAPD products

Muhammad A. Lodhi mal5 at cornell.edu
Wed Feb 2 10:22:35 EST 1994


Dear Friends
I have been trying to reamplify RAPD bands cut out of the agarose gel, with
the same primers as they were amplified the first time in RAPD reaction. 
In many cases I get more than one amplified product (bands) when I use a
single band as template.  Eventually, I will be labelling these bands with
digoxigenin once I am sure that I am getting only one product.  Has anyone
seen such an amplification (two bands from the amplification of a single
band) and what he/she did to overcome this problem.  Your suggestions will
be highly appreciated.  Thanks in advance

Muhammad A. Lodhi
Cornell University





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