Oligo design software

plxigpg at vax.ccc.nottingham.ac.uk plxigpg at vax.ccc.nottingham.ac.uk
Wed Jun 8 09:00:39 EST 1994


JANSEN WROTE:
>Does anyone know of a program for designing oligonucleotides for
>use as hybridisation or PCR probes on the basis of protein allignment

>and codon usage?

Well,

There is a program in the GCG packages called BACKTRANSLATE (available at
darsebury) which does exactly that ie. you give it an amino acid sequence
(most homologous regions of you alignment) , a codon usage table (some are
already provided eg.Ecoli) and it will output either the most probable
sequence or the most ambiguous (excuse the spelling) depending on what
you specify!!

Its a great little program!

Hope this helps,

Ian

plxigpg at vax.ccc.nottingham.ac.uk




More information about the Bio-soft mailing list