2nd Derivative Spectroscopy

Frederick Thum st93mey7 at dunx1.ocs.drexel.edu
Tue Sep 20 18:55:05 EST 1994


[ Article crossposted from bionet.software.sources ]
[ Author was Frederick Thum ]
[ Posted on 20 Sep 1994 18:49:32 -0400 ]


I am looking for software to run on a 286 interfaced via rs-232 with a
Hitachi U-2000 spectrophotometer.  The software must be capable of a
wavelength scan, finding the second derivative of the scan with a moving
least-squares algorithm, and finally identifying peaks and plotting the
amplitude of the peaks in a standard curve.

If anyone has a source for this type of software, please email me.

If you're curious why we need all this capability, we are doing nitrate
determination using a method described in the following article.  It shows
great promise with detection limits all the way down to 0.5 uM.

Crumpton, WG; Isenhart, TM; Mitchell, PD.  Nitrate and organic N analyses
with second-derivative spectroscopy.  Limnology and Oceanography, 37(4),
1992, 907-913.

Thanks in advance for any help.

- Fred Thum
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                Frederick L. Thum III   (st93mey7 at post.drexel.edu)
                    "Diatoms are as diatoms do." - Forrest Gump
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-- 
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
                Frederick L. Thum III   (st93mey7 at post.drexel.edu)
                    "Diatoms are as diatoms do." - Forrest Gump
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