Q: Primer design: Is there a source of statistical base distributions in genomes?
u7k0201 at sunmail.lrz-muenchen.de
Thu Aug 24 04:55:13 EST 1995
Primer sequences should only match to the targetted sequence. That sounds simple, but how to
prove it? OK, you can search the GenBank for another match, but the GenBank yet is not complete
(will it ever be?). I have heard about a method by mathematically deriving the probabilities of
certain sequences from the real distribution of small fragments (e.g dimers, tetramers or
hexamers) in a genome.
To compile these data sets I suppose there's a need of much workspace and machine capacity.
Thus I would like to know if this approach makes sense and is really neccessary and, if so,
if there already exists some data on the issue - where may it be found?
Thank you for your help!
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