A: Primer design: Is there a source of statistical base distributions in genomes?
u7k0201 at sunmail.lrz-muenchen.de
Fri Aug 25 08:27:31 EST 1995
sunger at crl.com (Stefan Unger) wrote:
>The MAC program RightPrimer already does this and is very reasonably
>priced. Send mailing address if you would like information. I am a dealer.
>(u7k0201 at sunmail.lrz-muenchen.de) wrote: : Hello everyone!
>: Primer sequences should only match to the targetted sequence. That sounds simple, but how to
>: prove it? OK, you can search the GenBank for another match, but the GenBank yet is not complete
>: (will it ever be?). I have heard about a method by mathematically deriving the probabilities of
>: certain sequences from the real distribution of small fragments (e.g dimers, tetramers or
>: hexamers) in a genome.
>: To compile these data sets I suppose there's a need of much workspace and machine capacity.
>: Thus I would like to know if this approach makes sense and is really neccessary and, if so,
>: if there already exists some data on the issue - where may it be found?
>: Thank you for your help!
>: Please email your posting, too.
>: Wolfgang Schechinger
>: [cut off, see below!]
>: (Standard disclaimer applies)
Dear Stefan, Thank you for your reply!
Testing my primer for another match on my gene is not the actual problem. Maybe I didn't point
that out clearly enough. I'm looking for a way of optimizing my primers for minimal match on
THE REST OF THE GENOME (OR mRNA/cDNA).
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email u7k0201 at sunmail.lrz-muenchen.de
(Standard disclaimer applies)
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