Isoelectric points?

rr at BMMB01.UMESCI.MAINE.EDU rr at BMMB01.UMESCI.MAINE.EDU
Thu Mar 2 15:08:56 EST 1995


Unless it is something you have to do frequently, it is easy to estimate by
hand, or if you want to get fancy, with a spreadsheet.  No estimate will be
better than the pKs you assume for the titratable groups and these are subject
to uncertainty. For starters, you could assume the following pKs, which come
from measurements by Nozaki and Tanford, which I don't have the exact reference
for at hand):
alpha-COOH 3.4
D 3.9
E 4.3
H 6.5
alpha-NH3 7.6
C 8.5 (although they could be in SS bonds-you wouldn't know)
Y 9.9
K 10.3
R 12.5


Good luck, Bob








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