ABI file reading apps?
Dr. P.L. Taylor
ptaylor at hgmp.mrc.ac.uk
Fri Mar 10 09:22:43 EST 1995
>I'm sure this has been asked before, but what software will allow you to
>open, view, manipulate ABI DNA sequencer Mac files? I understand DNAStar's
>Lasergene can, are there any others (GeneJockey II?), any freeware that
>will do this? Do ABI produce 'reader' software? Thanks.
>
>Fergus Doherty,
>dept. Biochemistry,
>University Medical School,
>Queen's Medical Centre,
>Nottingham NG7 2UH
>Tel: 602 709366 FAX 602 422225 Internet: mbxfd at unicorn.nott.ac.uk
GeneJockeyII opens chromatogram files and displays the contents, rather
better than ABI's own software. For example you can change all the colours
used (12% of the male population has deficient colour vision and most of
them have difficulty distinguishing between red and green - so what colours
do ABI choose for their traces?). You can edit the base calls, but not
save the data back to the original file, as GJII treats it as read-only.
GJII will also convert all the Ns to the full degenerate codes, and give
you a graph of predicted error rate against base number, which you can
use to select the 'good bit' of sequence to extract for alignment.
There's a demo at :
http://www.ebi.ac.uk/
ftp.ebi.ac.uk /pub/software/mac
Other software I know of which will open ABI files:
ABI's own programs : Analysis, SeqEd and Sequence Navigator.
DNAStar/LaserGene
MacVector.
GeneCodes' Sequencher.
Phil Taylor | MRC Reproductive Biology Unit
| Centre for Reproductive Biology
| 37 Chalmers Street
mbplt at seqnet.dl.ac.uk | Edinburgh EH3 9EW
ptaylor at hgmp.mrc.ac.uk | Scotland.
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