Evaluation of restriction sites

Gilles Truan truan at utsw.swmed.edu
Wed Nov 22 16:15:19 EST 1995


In article <48sku3$815 at mserv1.dl.ac.uk>,
   jpleiss at TEBIO1.BIOLOGIE.UNI-STUTTGART.DE (Juergen Pleiss) wrote:
>Dear colleagues,
>is there any programm available for the generation of restriction sites in
>a given nucleotide sequence? I have to introduce several restriction sites
>in a nucleotide sequence by changing the codon usage without altering 
>the amino acid sequence. Introducing these sites by hand is a very tediuos 
>work. So, I would like to know if someone knows a computer program doing 
>this work.
>Thank you,
>Juergen
	Yes Juergen, there is a little shareware for DOS called SILMUT, that 
you can find at ftp.bio.indiana.edu. It will take your aminoacid sequence and 
generate a map of possible restriction sites. You can edit the input file 
containing the restriction sites and enzymes names. If you need more 
information, just ask...

--------------------------------------------------------
Dr. Gilles TRUAN
Department of Biochemistry
University of Texas Southwestern Medical Center
5323 Harry Hines Blvd
Dallas, TEXAS 75206-9038
 Phone # : (214) 648-4741
   Fax # : (214) 648-8856
  e-mail : truan at utsw.swmed.edu
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