Introducing silent restriction sites

Christoph Gartmann GARTMANN at IMMUNBIO.MPG.DE
Tue Apr 2 04:39:23 EST 1996


In <315F9C11.507F at mrc-lmb.cam.ac.uk> Tony writes:

> we are looking for some (preferably Mac) software to identify the 
> positions where  'silent' restriction sites can be introduced by 
> mutagenesis into DNA sequence.

If you have access to GCG you may use MAP/SILENT (VMS) or map -silent (UNIX).
In conjunction with additional switches like /MAXCUTS=1 and the program MAPSORT
it shouldn't be to difficult to find a suitable restriticon site that can
easily be detected on a gel.

Regards,
   Christoph Gartmann


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