Free [Ca2++] in buffers

[From Jaume Canaves]

Hi!

 Does anybody know (or have) a program to calculate the concentration 
of free calcium in a solution that contains one or several kinds 
of calcium-binding molecules (EGTA, proteins, etc)?. I have seen a README 
file that describes such a program in several ftp locations, but I have 
been unable to locate either the executable program or the source code.
Any insight about such program or any other options will be very welcome...

Thanks!
										
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