Software/Method to determine an antisense RNA

Francois Jeanmougin pingouin at crystal.u-strasbg.fr
Tue Nov 5 08:17:22 EST 1996


In article <55nc61$q9e at c4.hrz.uni-giessen.de>,
	schlaude at paul.bio.uni-giessen.de (Gerd Schlauderer) writes:
>We want to construct an anti-sense RNA to suppress the expression of a
>protein. Is there a program to check, which sequence parts are not
>involved in stem-loops? 

>RNA-folding programs like mfold are not feasible for this problem,
>because the RNA is too large (about 5000 bases). Is it practable to run 
>fasta using a sequencewindow of 20 bases as query and the complementary 
>RNA-sequence as database? The lowest scoring sequences should be
>ideal for anti-sense RNA binding.

	Did you try a dotplot? just my 0.02.

						Francois.

-- 
Francois Jeanmougin
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