single chain antibodies - primer sequeneces?

Peter Kulmburg kulmburg at aug.ukl.uni-freiburg.de
Mon Oct 28 06:08:53 EST 1996


Dear collegues,

I will have to clone single chain Fv antibody fragments, and it is more
difficult than I thought it would be. Reading many publications is more
than confusing, any of them shows up with different primers, some are
degenerate, others are not, however, it always seems to work. Which primers
am I to take finally?

Is there anywhere a www page with sequence alignements or primer sequences
or something that might help me?

Thank you very much in advance!

Sincerely yours		Peter Kulmburg

Peter KULMBURG        at home  Tel./Fax:+49 761 80 84 39
at work:    Tel.: +49 761 270/7208 or 7207        Fax: /7217






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