Blast search help needed.
mperr1 at hamon.swmed.edu
Mon Sep 30 11:51:02 EST 1996
ckafer at iastate.edu (Chris Kafer) wrote:
>I would like to be able to input several Kb of DNA sequence and have a
>blast search against the protein databases and the EST database on all
>ORFs over 30 amino acids long in all 3 reading frames in each
>direction. Is there a simple way to do this?
Sounds like a job for blastx. Point your browser to the NCBI BLAST
web page at http://www.ncbi.nlm.nih.gov/BLAST/ and check out the
blastx: compares a nucleotide query sequence translated in all
reading frames against a protein sequence database
tblastx: compares the six-frame translations of a nucleotide query
sequence against the six-frame translations of a nucleotide sequence
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