Public-domain software available

Petr Kuzmic pkuzmic at biokin.com
Fri Aug 1 07:13:13 EST 1997


Program DYNAFIT [1] for the statistical analysis of enzyme kinetic and
receptor binding data can be downloaded the BioKin Ltd. web site [2].

The program is useful for fitting the following types of experimental
data:

 * initial reaction velocities in enzyme assays;
 * equilibrium composition of complex mixtures;
 * reaction progress (concentration or a related quantity vs. time).

The reaction mechanism is described symbolically, in the form of
chemical stoichiometric equations.  For example, tight-binding,
mixed-type, noncompetitive enzyme inhibition is notated as follows
(E...enzyme, S...substrate, I...inhibitor):

 [mechanism]
  E + S <===> ES
  ES ---> E + P
  E + I <===> EI
  ES + I <===> ESI
  ESI ---> EI + P

A reviewer [3] wrote: "Perhaps the most impressive feature is the
extensive set of sample script files that serves as a tutorial for
enzyme kinetics and receptor-ligand reactions. If you do any kind of
kinetic data analysis as part of your research program, be sure to
download DynaFit from the BioKin Web site."

REFERENCES

[1] Kuzmic, P. (1996) Anal. Biochem. 237, 260-273. "Program DYNAFIT for
the Analysis of Enzyme Kinetic Data: Application to  HIV Proteinase"

[2] http://www.biokin.com/dynafit/

[3] http://biomednet.com/hmsbeagle/1997/09/booksoft/salert.htm

------------------------+------------------------
Petr Kuzmic, Ph.D.      |          (608) 256-4790
BioKin Consulting       |      fax (708) 256-1269
P.O. Box 8336           |      pkuzmic at biokin.com
Madison WI 53708        |   http://www.biokin.com
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