Software available: enzyme kinetics and equilibria

Petr Kuzmic pkuzmic at biokin.com
Tue Aug 19 10:10:56 EST 1997


PUBLIC DOMAIN PROGRAM DYNAFIT UPDATED

Program DYNAFIT for the analysis and simulation of enzyme
kinetic and receptor binding data was updated to version
2.17.1 (08/18/97).  It has the capability to analyze multi-
response equilibrium binding data, where several dose res-
ponse curves are analyzed together, while each curve repre-
sents the equilibrium. concentration of a different species.

TEST PROBLEM: NUCLEASE/DNA BINDING MECHANISM

The new features of DYNAFIT are illustrated on complex 
equilibria involved in nuclease catalysis.  Two sets of 
gell-shift data (courtesy Dr. Paul Mitsis, seQ Ltd., Prince-
ton NJ), representing the concentration of (1) the protein
trimer-DNA complex and (2) the hexamer-DNA complex, were 
analyzed together.  The standard error of equibrium constants
was lower than if both datasets are analyzed separately.

LINKS

http://www.biokin.com/dynafit/download.shtml ... program
http://www.biokin.com/dynafit/downexam.shtml ... examples
http://www.biokin.com/dynafit/tutorial/nuclease.shtml

REFERENCES

P. Kuzmic (1996) Anal. Biochem. 237, 260-273.

_____________________________________________________________
Petr Kuzmic Ph.D. * BioKin Ltd. * Madison, WI 53708-8336, USA
pkuzmic at biokin.com * http://www.biokin.com * 608.256.1269 fax




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