Need to check my oligos/primers

ISOAI Atsushi aisoai at agc.co.jp
Wed Jun 11 08:10:07 EST 1997


In article <339976BE.56 at im.dtu.dk>, pwl at im.dtu.dk wrote:

>I've heard of an easy to use, small, freeware program, which is able to
>determine if a given PCR- or sequencing-primer is any good. 
>
>It should check inhibitory factors like dimers, stem-loop structures
>etc. which may impair proper hybridization.
...

Hi, Peter,
You can get a useful software "Amplify" for PCR from;
url: http://www.wisc.edu/genetics/CATG/amplify/index.html.
And I suggest good Web site; 
url: http://genome-www2.stanford.edu/cgi-bin/SGD/web-primer
for designing PCR and sequencing primers.
Please try. Good luck! Bye.

-- 
===================================================
Atsushi Isoai, Ph.D.    |   <aisoai at agc.co.jp>
Research Center, Asahi Glass Co.LTD., JAPAN
URL: http://www.yk.rim.or.jp/~aisoai/molbio-j.html
===================================================




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