Wanted: Calcium buffer program.

Athel athel at IR2CBM.CNRS-MRS.FR
Wed Jun 11 07:28:01 EST 1997


Len Bell asked:

>Does anyone know of a good program for calculating free calcium (Ca2+) levels in buffers containing [ATP/ADP], [EGTA], pH etc.

Your colleague John Easterby (jse at liverpool.ac.uk) should be able to
help you on this one! Take a look at his pages at
http://www.liv.ac.uk/~jse/software.html and
http://www.bi.umist.ac.uk/Staff/Academia/RJB/Buffers/Bufbook.html.

I haven't yet looked at his book myself, but I read a very complimentary
review of it recently. It seems to be mainly (or exclusively) concerned
with pH, but the same principles apply to pCa, and I don't see any
obvious reason why the program couldn't be modified to calculate pCa.

My own program described in Biochem. J. (1976) 159, 1-5 could certainly
do these calculations, but as it is a Fortran program for running on
mainframes (and not at all friendly by modern standards), I doubt if it
would satisfy you. In any case, I'm not sure how easily I could dig out
a copy. I mention it here only because it wouldn't be especially
difficult to rewrite it to modern standards, and I could probably be
persuaded to do it if there were a demand.

Athel





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