Software to locate mutations in a known sequence?

[John Little]

We isolate lots of mutants in a sequenced organism (phage lambda), and
would like to find software in which we can have a "template" showing
the wild-type sequence, with multiple landmarks including genes, to
which we can compare the sequence of a mutant.  Ideally, it would
identify any changes, then tell us either 1. which gene is altered and
the amino acid change or 2. which cis-acting site is changed.  Any
suggestions for such software?

John Little
Department of Biochemistry
University of Arizona
jlittle at u.arizona.edu