DynaFit 3.16 available ([bio]chemical kinetics and equilibria)

Petr Kuzmic pkuzmic at biokin.com
Mon Feb 15 23:40:11 EST 1999


Program DynaFit [P. Kuzmic (1996) Anal. Biochem. 237, 260-273]
for Windows-95/NT was updated to version 3.16.

Among other improvements in the analysis of enzyme kinetic data,
you can now plot reaction velocities in the Lineweaver-Burk 
coordinates (1/[substrate] vs. 1/velocity).

An example script file using this feature is located in

  ./examples/Segel/Ch-IX/N/Rapid Equilibrium Random Bi Bi.txt

Here is a listing of this script, corresponding to the reaction
scheme from I. Segel (1975) "Enzyme Kinetics", page 643:

----------- 8< ---------- 8< --------------
[task]

   task = simulate
   data = velocities

[mechanism]

   E + A <==> EA        :   Kia    dissoc.
   E + B <==> EB        :   Kib    dissoc.
   EA + B <==> EAB      :   Kmb    dissoc.
   EB + A <==> EAB      :   Kma    dissoc.

   EAB ---> E + P + Q   :   kp

   E + P <==> EP        :   Kip    dissoc.
   E + Q <==> EQ        :   Kiq    dissoc.
   EP + Q <==> EPQ      :   Kmq    dissoc.
   EQ + P <==> EPQ      :   Kmp    dissoc.

   EPQ ---> E + A + B   :   k-p

[constants]

   Kia = 1, Kib = 1, Kma = 1, Kmb = 1
   kp  = 1

   Kip = 1, Kiq = 1, Kmp = 1, Kmq = 1
   k-p = 1

[concentrations]

   E = 0.001
   A = 10

[responses]

   P = 1

[progress]

   rapid equilibrium

[velocity]

   directory  ./examples/segel/ch-ix/n/data
   extension  txt
   mesh       from 2 to 100 step 2 logarithmic
   error      constant 0.00002
   variable   B
   plot       Lineweaver-Burk

   file p0 | concentration P = 0
   file p1 | concentration P = 10
   file p2 | concentration P = 20
   file p3 | concentration P = 40
   file p4 | concentration P = 80
   file p5 | concentration P = 160

[output]

   directory  ./examples/segel/ch-ix/n/output

[end]
----------- 8< ---------- 8< --------------

Dowload the program from URL below...

_____________________________________________________________
Petr Kuzmic Ph.D. * BioKin Ltd. * Madison, WI 53708-8336, USA
pkuzmic at biokin.com * http://www.biokin.com * 608.256.1269 fax




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