PCR consensus primer design

Thomas Burmeister tbu at gmx.net
Fri Jan 22 08:54:20 EST 1999


Hello !

I have aligned a set of about 50 related DNA-sequences and want to
create a set of consensus primers (one direction)  that should be able
to amplify (nearly) every single sequence in this alignment. There
should not be to much primers (<10) but of course the degeneracy of
each of them should also be not too high.
Does anyone know of a computer program that is able to perform this?

Thanks in advance for any help (please mail a copy of your answer to:
tbu at gmx.net) !

Thomas Burmeister







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