Measureing an area
sschmid2 at Uni-MolGen.gwdg.de
Mon Jun 14 05:39:15 EST 1999
"David B. Hedrick" wrote:
> Wow. What software do you use?
> You could blow up the image, print it out, cut out the cricket wing
> cell, and weigh the piece of paper. Now, stop laughing, I'm serious.
> Do the same for something you took an EM of and that you know the area
> of to determine the scale. Cut out and weight 5 copies of the same
> image to determine the variation in your method. Maybe try different
> types of paper to find one that is constant. Regular lazer jet paper
> works fine and is cheap. If this cricket wing business is something
> you're going to do once and quit, this quick and dirty method might get
> you done quicker than something more computerized.
Thanks for that straight forward solution :-).
At the moment I am simplifying the image in black and white. Then I measure with
the pick-tool of Corel Photo-Paint the whole picture. The grey-value gives me a
value of 0-255 which I then normalize to 100 %. With the help of the EM-scale I
can determine the size of the wing.
A friend of mine suggested to convert the picture into an ASCII-file with 'xv'
(black-and-white) and then counting the black pixel by the command grep '255'
datei | wc.
> Or, Visual basic (and almost all other programming languages) will let
> you determine the color of a pixel of a bitmap. You could use Corel
> Photo-Paint or Paint Shop Pro to simplify the image - 2 color only, and
> use the magic wand to copy the cell to a blank bitmap. Then a very
> simple program could count the colored pixels. You could even have it
> color each pixel as it is counted so you can see that it is working
> Or, when you increase the magnification of an image in Paint Shop Pro
> to the maximum, you can clearly see the individual pixels. You could
> count them yourself. Use a drawing tool with a color not used in your
> image to mark out each one as you count.
> Or, you could hire someone like, well, like me to do it for you.
> Have fun!
> > Hi,
> > I want to measure the size of a cell on a crickets'wing. Therefore I use
> > an EM and save the picture as a bitmap. Has anyone an idea how to
> > measure an area in a bitmap-picture? It would be appreciable when there
> > would be a tool which can automatically select the wanted area - like
> > the magic wand mask tool of Corel Photo-Paint.
> > Thanks
> > Steffen
> > ---------------------------------------------------------------
> > Name: sschmid2.vcf
> > Part 1.2 Type: text/x-vcard
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> > Description: Card for Steffen Schmidt
> Technical writing, literature search, and data analysis at the interface
> of chemistry and biology.
> davidbhedrick at icx.net
> David B. Hedrick
> P.O. Box 16082
> Knoxville, TN 37996
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