Local BLAST search

[Tom Hall]

You can do this as easily as a couple of mouse clicks with a freeware
program called BioEdit.  It's a little big, but it installs itself and
costs $0.
BioEdit can be downloaded anonymously, installs itself, and allows you
to automatically create local NCBI nucleic acid and protein databases
and run local (or internet) Blast searches on sequences from a doc
window simply by highlighting the sequence title and clicking a menu
option.  All the standard Blast programs are supported (blastp, blastn,
tblastn, blastx).  It's also a fully-functional alignment editor that
reads and writes multiple formats, reads ABI trace files, does nice
shaded outputs, does several sequence manipulations, automatically runs
accessory apps, auto-links to the web, and has several other useful
features.

If you want to try it out, it's at:

http://www.mbio.ncsu.edu/RNaseP/info/programs/BIOEDIT/bioedit.html

If you don't like it, it uninstalls itself like any other Windows95/98
program.

Hope this helps,

Tom Hall

David Parkin wrote:

> Dear all,
>
> I'm working with Windows versions of Lasergene and
> Vector NTI packages. I would like to know if it is
> possible to "learn" them (or olny one of them) to
> search not an Internet BLAST database at NCBI, but
> local database established with NCBI soft (blastz.exe)
> and my own sequences how it makes DNA Tools package.
> If possible, please write me how.
>
> With best wishes,
>
> David
>
> =====
>
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