ABI data extraction utilities

Gys de Jongh GysdeJongh at compuserve.com
Wed Apr 5 16:57:44 EST 2000


"Przemko Tylzanowski" <przemko at med.kuleuven.ac.be
> wrote in message news:38EB475D.B546C7D3 at med.kuleuven.ac.be...
>I use Chromas (the freeware version) and am very happy with it.
> The thing is that sometimes I would like to see the raw data 
>because the ABI sometimes miscalls the bases. Now, our lab 
>is PC based and the only Mac is with ABI. So it would be 
>much more convenient to look at the raw data on PC. I have
>somewhere a hack for it but I thought perhaps someone 
>managed a program to read not only
>the processed data but also the real trace.
>"Richard J. Dudley" wrote:
>Have you tried Chromas?

>http://www.technelysium.com.au/chromas.html
>or BioEdit?

>http://www.mbio.ncsu.edu/RNaseP/info/programs/BIOEDIT/bioedit.html

Hi,
there is a pc version for chromas which works very nice. 
It lets you view , edit , save the ABI called bases. If you choose
"export" in the "file" menue it will save the raw fluorescent data.

Bioedit can do the same ; it will,however, not save the raw flu. data.

I also tried exTRACE of Mark Berres. I found it very usefull as
it extracts all the data , not only the raw fluorescense.
(yes Richard , there is a lot more in the ABI file) It works
perfectly in a dos window as he describes in this thread.

-- 
Gys









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