PC software to create restriction site maps

Karsten Karla karla at uni-muenster.de
Wed Apr 12 11:35:59 EST 2000


Hello to everyone,

some weaks ago I have isolated a new plasmid from a bacterial strain and now I´m trying to carry out a characterisation of this plasmid. There´s quiete a number of enzymes which cut and I also did a number of double digestions. The problem is now to combine the fragments to a corresponding map. Unfortunately the plasmid is to large (20 kB) to sequence. Does anyone have an idea about PC-software which helps in the case that there is no further information about the sequence of the DNA? I found some files concerning a programme called "double digester" but the attempt to install it on my PC failed.

I would be very pleased if anyone could give me a hint

Thanks

Karsten



Karsten Karla

Universitaet Muenster
Institut f. Biochemie
Wilhelm-Klemm-Strasse 2
D-48149 Muenster

Tel.:+49/251/83-33040
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