Primer design from multiple alignment.

[Bill_A_Nussbaumer at ms.bd.com]

Bill A Nussbaumer at BDX
01/24/2000 03:09 PM

Hi all,

I'm trying to find a computer program, preferably Win32 compatible but possibly
Linux compatible that will identify optimal primer sequences for PCR based on a
multiple sequence alignment.  i.e.  If I align a set of sequences from a single
genus that have some variation, I would like to find the best primers to amplify
all members of the set.  Any ideas would be most appreciated.

TIA,

Bill Nussbaumer


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