New options to analyze Human GENOME by Genome Explorer
webmaster at softberry.com
Wed May 16 04:44:55 EST 2001
New options to analyze Human GENOME by Genome Explorer
--------- 2nd version of Softberry Genome Explorer INCLUDES Search possibility to
find location of EST/mRNA in genome if you know its AC GenBank and tools for
Extracting and visualization sequences of genes and their coding regions
Softberry Genome Explorer allows visualization and sequence extraction of many
genome features, such as known and predicted genes, mRNAs, EST, known genes
and many others, on large genomic sequences.
On this server, Genome Explorer shows these features for human genome draft
from UCSC Human Genome Project Team.
In addition to publicly available information, Genome Explorer includes genes
predicted by Softberry's FGENESH++ program. Genome Explorer can be used to
extract nucleotide and protein sequences of particular genes, functional sites and
other features. These sequences can be viewed by right-clicking features of
interest in Gene Explorer's main window.
Searching Genome for Feature of Interest
Genome Explorer has search function that help you visualize feature of interest
on a genome. Features can be searched by GenBank AC number (mRNAs and
known genes), EST ID, cytogenetic band name, repeat name or FGENESH++
predicted gene ID. Search can be performed only on feature types loaded into
memory (Options > Load Feature Types option, see below). To start search,
press FIND FEATURE button or select Search > Find Feature option from a
menu. Find Feature dialog is displayed, where you can specify search conditions.
After search was performed, a list of found features is displayed. To view a
feature and its surroundings on genome map, click on a feature from the list and
press LOAD button. List of found features is stored in memory until new search is
performed or Genome Explorer is exited.
Selecting Chromosome Region.
Chromosome can be selected from Chromosome Selection Dialog Box. To open
it, either choose Options> Chromosome from main menu or press Chromosome
button. In Chromosome Selection Dialog Box, select chromosome from pull-
down list. Then select chromosome region to be loaded. It can be done using a
ruler just below the pull-down list, or by entering selected sequence boundaries'
positions in fields FROM, TO and/or SIZE. By default, a 600-kb fragment of
chromosome 1 is loaded. Please keep in mind that the larger selected region, the
longer it will take to load and the slower to navigate.
The ruler is a graphical representation of entire given chromosome. You can
choose region to load by moving and reshaping selection (green shaded
rectangle) along its length:
- Dragging selected region by mouse to the left or to the right;
- Dragging one of the region's boundaries;
- Shift-dragging region's boundary - in this case both boundaries
expand or retract to the same extent.
Boundaries of selected region are displayed in fields FROM and TO below
chromosome's map, and its size in SIZE field. To select by sequence position,
enter start and end positions in the fields FROM and TO, or start (end) position
and length in fields FROM (TO) and SIZE. Press ENTER key or move cursor to
another field to record entered values. If entered values in any of the fields go
beyond chromosome's boundaries, they are corrected and new values are
displayed in the fields. Changing value in one of the field may result in change in
another - e.g. entering new value in FROM will change value in SIZE. Selected
region may be shifted to the left or to the right by pressing buttons <<LEFT and
RIGHT>>. The size of the shift must be entered in the field between these two
buttons. Default value is 10 MB. After selection is made, pressing OK closes
Chromosome Selection Dialog Box and displays part of selected region in
Explorer's main window.
Selecting Features to Display.
Options > Load Feature Types menu option allows you select features to be
loaded in computer memory. Of these loaded, some or all can be displayed in
Genome Explorer window. Selecting Features to display can be done by either
pressing FEATURES button or choosing Options> Features from main menu.
The following features can be selected:
Gap locations (on nucleotide sequence)
Golden Path Positions of chromosome segments
Gold chromosome assembly
Genes predicted by FGENESH++ program
Known genes (from GenBank/Infogen entries)
Known mRNA sequences
Known EST sequences
SNPs from clone overlaps
SNPs from random reads
Sequence Tag Marker locations
Mouse syntheny regions
Chromosome cytogenetic band locations.
By default all features are selected in both LOAD FEATURES TYPES and
FEATURES dialogs. Search can be performed only among loaded features.
Navigating Main Window
After choosing chromosome region and features, a fragment of loaded region is
displayed in the main window. You can change size and location of displayed
fragment using ruler and boundary/size fields the same way you did it in
Chromosome Selection Dialog Box. But in the main window, a ruler represents
loaded chromosome region rather than whole chromosome.
You can also move along the region's length by dragging main window map by
mouse to the left or to the right. Shift-drag to the left decreases map resolution,
while same move to the right increases it. If option "Repaint on Drag" is enabled
(default), the map moves along with mouse. If it is disabled, map is redrawn only
after mouse button is released. Finally, you can choose a region on the map by
Ctrl-dragging, and this region will expand over whole window. Maximal resolution
of Genome Explorer is one base pair per pixel.
Features of selected region are displayed in the middle section of main window.
Each feature type is shown on its own line(s). By default, if features of same type
overlap on sequence, they are shown on separate lines, while top line for each
type of features (shaded in gray) shows all features of that type combined. You
can switch off display of combined lines or separate lines by chosing Options >
Feature Show Mode > All Objects or > Combined Objects.
When mouse pointer touches some feature on the map, it changes its shape to a
hand. At that time, if option "Show Information on Mouse Over" is enabled
(default), available information on each feature is shown in left bottom panel
(Feature Info Panel). If that option is disabled, you must click left button to get
information displayed. If mouse pointer is located over multi-block (multi-exon)
feature, like gene or EST with several alighnment blocks, Feature Info Panel is
divided into two sections. In left section, information about whole section is
displayed, while in right section, information about selected (pointed to) block.
On top of main window's ruler, there are buttons "Back" and "Forward" which
operate in a way similar to same buttons in Internet browsers.
Mouse right-clicking anywhere on the map opens a menu with the following
Show Sequence - (+) and (-) - Shows nucleotide sequence around
the point clicked, positive or negative strand.
Show Feature Sequence - (+) and (-) - Shows nucleotide sequence
of a given feature on either of two strands, as well as short flanks.
Enabled only when pointer is located over a feature (hand-shaped).
Show Block Sequence - (+) or (-) - shows nucleotide sequence of a
given block (exon).
Show Protein - Shows amino acid sequence of gene product.
Enabled only if gene is pointer is located over a gene.
Show Entry - Shows GenBank entry for mRNA or EST.
Sequences and entries are shown in a separate window. Options of sequence
display are determined by Sequence Options menu (Options>Sequence).
Sequences of selected features are shown in bold capital letters, while flanks are
shown in lowercase. Exons on exon-containing features (gene, mRNA, EST), are
highlighted in blue. Selected exons, if any, are shown in red.
More information about the Bio-soft