Signal Peptide Predictions

Nicholas Dickens nicholas.dickens at human-anatomy.oxford.ac.uk
Fri Oct 26 04:57:20 EST 2001


I have used a bioperl module called sigcleave.pm, and compared it
with the signal P web server results, see:

 http://www.cbs.dtu.dk/services/SignalP-2.0/

If there are discrepancies I recommend the signalP2 results over
sigcleave. This is all PC based stuff, bioperl requires perl, in
linux this is easy, for windows google search for activeperl (free
from activestate but I can't remeber the url).

Hope this is useful.

Regards

Nick

"Mr. A. Brown" wrote:
> 
> Hi.
> 
> I have a small database of mammalian signal peptide sequences. I am
> looking for a method (program) that would allow me to scan these signal
> peptides, in conjuction with a protein I wish to express, so that I can
> select the 'best' signal peptide for that protein. The proteins are to
> be expressed in both COS and CHO cells (hopefully secreted).
> 
> We run both Macs and PC's in our lab, so programs for either (or both)
> would be welcome, as would web-based systems (Netscape or Explorer).
> 
> Many Thanks in Advance.
> 
> Alex Brown,
> e-mail: abrown at nimr.mrc.ac.uk
> e-mail: abrown at hgmp.mrc.ac.uk

-- 
Nicholas Dickens BSc ARCS

MRC FGU, Department of Human Anatomy & Genetics,
South Parks Road, Oxford, OX1 3QX
Tel: 01865 2(82650)
http://www.ndickens.fsnet.co.uk/
nicholas.dickens at human-anatomy.ox.ac.uk





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