please post this mail in your news group - thank you in advance !!!

Dr. Michael Pfaffl genequan at
Thu Apr 4 05:29:53 EST 2002

Main focus of GENE QUANTIFICATION web page is to describe
and summarize all technical aspects involved in quantitative
gene expression analysis using real-time (RT-) PCR.
( specificity, sensitivity, reproducibility, intra- and inter-assay 
variations, etc.)

The GENE QUANTIFICATION web page illustrates
the usefulness of a reliable quantification strategy,
and the difference between absolute vs. relative quantification
assays in real-time (RT-) PCR.

RT-PCR is the technique of choice for analysing mRNA in extremely low 
Real-time RT-PCR using SYBR Green I detection combines the ease
and necessary exactness to be able to produce reliable as well as rapid 
To obtain high accuracy and reliability in RT and real-time PCR highly 
quantification strategies and methods are needed.  content:

- Real-time (RT-) PCR  and  optimisation !!!
- Available  real-time PCR  platforms
- Description  of  quantification  strategies  in  real-time (RT-) PCR
- Description  of  absolute quantification  and  relative quantification
- REST © ( Relative  Expression  Software  Tool © )
- Housekeeping Gene  problematic  in  real-time PCR  ( under construction !! )
- Verification of array results via kinetic RT-PCR  ( under construction !! )
- Comparison  of   real-time RT-PCR   versus   competitive RT-PCR
- Download  of  software  tools  (e.g.  REST © )
- Summary  of  relevant  literature
- Summary  of  relevant  links

for further information contact Dr. Michael W. Pfaffl at

Gene Quantification @

e-mail:         gene.quantification at

responsible Senior Scientist:
Dr. Michael W. Pfaffl
Institute of Physiology
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan

-------------- next part --------------
An HTML attachment was scrubbed...

More information about the Bio-soft mailing list