Software to design new restriction site via point mutation

Jaco jaco.de.ridder at bioagric.up.ac.za
Mon Jan 14 04:27:04 EST 2002


> I would be very happy , if you could help me with the following problem:
> 
> I need to design a new restriction site anywhere in a given protein. I have
> 10 non-cutters.
> 
> I need a Software, which can show me all the sites in which i could create
> a new restriction site (of one of the 10 non cutting proteins) using a
> single base silent point mutation.
> 
> Can you tell me which software can do that? Is there even free software
> that can do it?

You might like to look at Webcutter! 
(http://www.firstmarket.com/cutter/cut2.html)

Amongst others it can determine "Silent cutters" - Find sites which may
be introduced by silent mutagenesis of your coding sequence... 

-- 
Jaco de Ridder         | Tel +27 12 420 4149
Dept Biochemie         | Fax +27 12 362 5302
Universiteit van Pretoria, RSA
E-mail: jaco.de.ridder at bioagric.up.ac.za

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