Primer design

Charlie cckim at stanford.edu
Wed Jun 12 11:27:14 EST 2002


Primer3.  Two thumbs up.  Simple, yet lots of features if you want them.

Charlie

On 12 Jun 2002, Chris Upton wrote:

>
> Hi,
>   I'm looking for a simple PCR primer design program.
>
> I want to amplify a series of genes, so my primers are pretty much
> fixed in position.
> I want to calculate Tm and look for potential hairpins and primer
> dimers from my pairs of primers.
>  But I don't want to have to enter the target sequence for all of these.
>
> Any suggestions?
>
> Thanks,
>   Chris
>
>





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