Questions about the primer design software usage

Jack Lee wleemd at mail.edu.cn
Thu Feb 26 10:31:27 EST 2004


I tried the "Primer3" software (web based edition) to test a pair of primers I
found on a paper, but always got the output:"specified left/right primer not found
in the sequence."
What's the wrong here?
I searched the web, and found an introduction to "Primer3", it mentioned forward
and reverse primer, but in papers "forward and reverse primer" never occured.
Some papers only gave:
"Primer A 5' agttca......ccagtatt 3'
        B 5' ccagta......cagattat 3'"
Some said:
"Primer 5' agttca......ccagtatt
        3' ccagta......cagattat
So which is the forward primer, which is the reverse primer?

I also tried the "Primer Premier", and experienced the same problem.



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