[Bio-software] error in run blastpgp

hamze rahimi via bio-soft%40net.bio.net (by buksanbio from gmail.com)
Tue Jan 4 06:38:12 EST 2011


Dear expert

I download blast-2.2.18-ia32-linux.tar.gz and unzipped. when I want run
under command, I find this error

[buksanbio from localhost bin]$ ./blastpgp -v 5000 -b 0 -j 3 -h 0.002 -d
/home/buksanbio/nr/ -i 535.fasta -C.Chk  > /dev/null
[blastpgp] WARNING: Unable to open .pin

or

[buksanbio from localhost bin]$ ./blastpgp -v 5000 -b 0 -j 3 -h 0.002 -d ~/nr/*
-i 535.fasta -C.Chk  > /dev/null
[blastpgp] ERROR: Arguments must start with '-' (the offending argument #11
was: '/home/buksanbio/nrfilt/nr.00.pin')

as recommanded  in doc file I convert database by this command:
./formatdb -i ~/.../nr -p T -o T


nr is my database that contain these files:
nr.00.phr  nr.00.psd  nr.01.pin  nr.01.psi  nr.02.pnd  nr.02.psq  nr.03.pni
nr.04.phr  nr.04.psd
nr.00.pin  nr.00.psi  nr.01.pnd  nr.01.psq  nr.02.pni  nr.03.phr  nr.03.psd
nr.04.pin  nr.04.psi
nr.00.pnd  nr.00.psq  nr.01.pni  nr.02.phr  nr.02.psd  nr.03.pin  nr.03.psi
nr.04.pnd  nr.04.psq
nr.00.pni  nr.01.phr  nr.01.psd  nr.02.pin  nr.02.psi  nr.03.pnd  nr.03.psq
nr.04.pni  nr.pal

what is problem?
thanks a lot for any help


-- 
Yours sincerely
Rahimi Hamzeh
PhD student in Medical Biotechnology
Pasteur institute
e mail:
buksanbio from gmail.com


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